Annual dynamics of eukaryotic and bacterial communities revealed by 18S and 16S rRNA metabarcoding in the coastal ecosystem of Sagami Bay, Japan

Sagami Bay, Japan is influenced by both the warm Kuroshio Current and cold Oyashio rich nutrients are supplied from multiple river sources deep-sea, forming a dynamic ecosystem. The aim of present study was to investigate eukaryotic bacterial communities in coastal waters using 16S rRNA 18S sequencing assess seasonal vertical dynamics relation physicochemical biological conditions. Eukaryotic showed synchronous changes along with environmental variability. Diversity plankton community suspended surface lower than those at subsurface layers eukaryotes bacteria communities; however, diversity different characteristics where deeper layer (100 m) as low highest intermediate depth (10–50 m), while that m). annual variability microbial driven, not only abiotic biotic factors short-term rapid water inflow phytoplankton blooms, but also largely deep-seawater upwellings due unique seafloor topography.